Contenidos del Libro:
- Radioligand-Binding Methods for Membrane Preparations and Intact Cells
- Determination of Allosteric Interactions Using Radioligand-Binding Techniques
- Generation, Use, and Validation of Receptor-Selective Antibodies
- Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization
- Generation and Use of Epitope-Tagged Receptors
- Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization
- Electron Microscopic Studies of Receptor Localization
- Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
- Viral Infection Protocols
- Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection
- Covalent Modification of G-Proteins by Affinity Labeling
- Measurement of Agonist-Stimulated [35S]GTPγS Binding to Assess Total G-Protein and Gα-Subtype-Specific Activation by G-Protein-Coupled Receptors
- Identification and Quantitation of G-Protein α-Subunits
- Analysis of Function of Receptor–G-Protein and Receptor–RGS Fusion Proteins
- Assessment of Receptor Internalization and Recycling
- G-Protein-Coupled Receptor Phosphorylation and Palmitoylation
- Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping
- Ubiquitination of G-Protein-Coupled Receptors
- Receptor Mutagenesis Strategies for Examination of Structure–Function Relationships
- Study of G-Protein-Coupled Receptor–Protein Interactions by Bioluminescence Resonance Energy Transfer
- Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells
- Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation
- Study of G-Protein-Coupled Receptor–Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase–Fusion Protein Pull-Downs
- Receptor Knock-Out and Knock-In Strategies
- Statistical Methods in G-Protein-Coupled Receptor Research
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