Crystallography Made Crystal Clear: A Guide for Users of Macromolecular Models

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Último libro que subo sobre proteínas y cristalografía (tuve certamen de esto así que me rayé un poco). Ahora si quieren puedo buscar libros sobre NMR para sanjar el tema... les dejo la decisión a ustedes.
Saludos.

Contenidos del Libro:

1. Model and Molecule
2. An Overview of Protein Crystallography
3. Protein Crystals
4. Collecting Diffraction Data
5. From Diffraction Data to Electron Density
6. Obtaining Phases
7. Obtaining and Judging the Molecular Model
8. A User’s Guide to Crystallographic Models
9. Other Diffraction Methods
10. Other Kinds of Macromolecular Models
11. Tools for Studying Macromolecule
    

Membrane Protein Purification and Crystallization: A Practical Guide

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Contenidos del Libro:

Part I: Strategies and Techniques
1.- Purification Strategies for Membrane Proteins
2.- Techniques and Basic Operations in Membrane Protein Purification
3.- Production and Purification of Recombinant Membrane Proteins
4.- SDS Electrophoresis Techniques
5.- Blue Native Electrophoresis
6.- Preparative Isoelectric Focusing
7.- Membrane Protein Crystallization

Part II: Discussion of Selected Isolation Protocols
8.- Lipid-Dependent Inactivation and Reactivation of Bovine Complex III
9.- Purification of an Affinity-Epitope Tagged G-Protein Coupled Receptor
10.- Purification of NhaA Na+/H+ Antiporter of Escherichia Coli for 3D or 2D Crystallization
11.- Purification of the Cytochrome bc1 Complex from Yeast

Parte III: Crystallization of Membrane Protein
12.- Antibody Fragment Mediated Crystallization of Membrane Proteins
13.- Crystallization of Wolinella succinogenes Quinol: Fumarate Reductase
14.- Ba3-Type Cytochrome c Oxidase from Thermus thermophilus: Purification, Crystallization, and Crystal Transformation
15.- Two-Dimensional Crystallization of Membrane Proteins: A Practical Guide
16.- In Cubo Crystallization of Membrane Proteins

Structural Biology of Membrane Proteins

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Contenidos del Libro:

Seccion I: Expression and Purification of Membrane Proteins
1.- Refolding of G-Protein-Coupled Receptors

2.- Expression of Genes Encoding Eukaryotic Membrane Proteins in Mammalian Cells
3.- Expression of Recombinant G-Protein Coupled Receptors for Structural Biology
4.- The Purification of G-Protein Coupled Receptors for Crystallization
5.- An Introduction to Detergents and Their Use in Membrane Protein Studies

Seccion II: Methods for Structural Characterization of Membrane Proteins
6.- Solution NMR Approaches to the Structure and Dynamics of Integral Membrane Proteins
7.- Membrane Proteins Studied by Solid-State NMR
8.- Assessing Structure and Dynamics of Native Membrane Proteins
9.- State-of-the-Art Methods in Electron Microscopy, including Single-Particle Analysis
10.- Atomic Resolution Structures of Integral Membrane Proteins Using Cubic Lipid Phase Crystallization

Seccion III: New Membrane Protein Structures
11.- Aquaporins: Integral Membrane Channel Proteins
12.- Gas Channels for Ammonia
13.- Channels in the Outer Membrane of Mycobacter
14.- The Structure of the SecY Protein Translocation Channel
15.- Structure and Function of the Translocator Domain of Bacterial Autotransporters
16.- X-Ray Crystallographic Structures of Sarcoplasmic Reticulum Ca2ϩ-ATPase at the Atomic Level
17.- Comparison of the Multidrug Transporter EmrE Structures Determined by Electron Cryomicroscopy and X-ray Crystallography
18.- Structure of Photosystems I and II
19.- Glutamate Receptor Ion Channels: Structural Insights into Molecular Mechanisms
20.- The Mitochondrial ADP/ATP Carrier

Structural Bioinformatics of Membrane Proteins

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Sigo con las proteínas... espero que no me encuentren monotemática.
Contenidos del Libro:

1. Evolutionary origins of membrane proteins
2. Molecular archeological studies of transmembrane transport systems
3. Resource for structure related information on transmembrane proteins
4. Topology prediction of membrane proteins: how distantly related homologs come into play
5. Transmembrane beta-barrel protein structure prediction
6. Multiple alignment of transmembrane protein sequences
7. Prediction of re-entrant regions and other structural features beyond traditional topology models
8. Dual-topology: one sequence, two topologies
9. Predicting the burial/exposure status of transmembrane residues in helical membrane proteins
10. Helix–helix interaction patterns in membrane proteins
11. Predicting residue and helix contacts in membrane proteins
12. Natural constraints, folding, motion, and structural stability in transmembrane helical proteins
13. Prediction of three-dimensional transmembrane helical protein structures
14. GPCRs: past, present, and future

Actualización: 01/09/2012

Protein Nanotechnology: Protocols, Instrumentation and Applications

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Contenidos del Libro:

1. Protein Nanotechnology: The New Frontier in Biosciences
2. Kinetics and Mechanisms of Protein Crystallization at the Molecular Level
3. Nanostructured Systems for Biological Materials
4. Nanomaterials of Drug Delivery Systems for Tissue Regeneration
5. Nanotechnology With S-Layer Proteins
6. Folding of β-Structured Fibrous Proteins and Self-Assembling Peptides
7. Application of NMR Methods to Identify Detection Reagents for Use in Development of Robust Nanosensors
8. Studying 3D Subdomains of Proteins at the Nanometer Scale Using Fluorescence Spectroscopy
9. Carbon Nanotubes and Nanowires for Biological Sensing
10. Carbon Nanotube Systems to Communicate With Enzymes
11. Molecularly Imprinted Polymers for Biomolecular Recognition
12. Plasmonics-Based Nanostructures for Surface-Enhanced Raman Scattering Bioanalysis
13. Bacterial Virus φ29 DNA-Packaging Motor and Its Potential Applications in Gene Therapy and Nanotechnology
14. Construction of Ordered Protein Arrays
15. Bioengineering and Characterization of DNA–Protein Assemblies Floating
16. on Supported Membranes
17. Nanosystems for Biosensing: Multianalyte Immunoassay on a Protein Chip
18. Optical Nanosensors for Detecting Proteins and Biomarkers in Individual Living Cells
19. Nanoelectrodes Integrated in Atomic Force Microscopy Cantilevers for Imaging of In Situ Enzyme Activity
20. Protein Amyloidose Misfolding: Mechanisms, Detection, and Pathological Implications
21. Near-Field Scanning Optical Microscopy for Bioanalysis at Nanometer Resolution

Actualización: 23/08/2012

Protein-ligand Interaction

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Contenidos del Libro:

1. Isothermal Titration Calorimetry
2. Direct Optical Detection of Protein-Ligand Interactions
3. Label-Free Detection of Protein-Ligand Interactions by the Quartz Crystal Microbalance
4. Measurement of Solvent Accessibility at Protein-Protein Interfaces
5. Hydrophobic Interaction Chromatography: Harnessing Multivalent Protein–Surface Interactions for Purification Procedures
6. Sedimentation Velocity Method in the Analytical Ultracentrifuge for the Study of Protein-Protein Interactions
7. Protein-Ligand Interaction Probed by Time-Resolved Crystallography
8. X-Ray Crystallography of Protein-Ligand Interactions
9. Combined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in Metalloproteins
10. NMR Studies of Protein-Ligand Interactions
11. Probing Heme Protein-Ligand Interactions by UV/Visible Absorption Spectroscopy
12. Ultrafast Time-Resolved IR Studies of Protein-Ligand Interactions
13. Monitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference Spectroscopy
14. Proteins in Motion: Resonance Raman Spectroscopy as a Probe of Functional Intermediates
15. Fluorescence Polarization/Anisotropy Approaches to Study Protein-Ligand Interactions: Effects of Errors and Uncertainties
16. Ligand Binding With Stopped-Flow Rapid Mixing
17. Circular Dichroism Spectroscopy for the Study of Protein-Ligand
18. Interactions
19. High-Throughput Screening of Interactions Between G Protein-Coupled Receptors and Ligands Using Confocal Optics Microscopy
20. Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics
21. Application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding
22. Atomic Force Microscopy Measurements of Protein-Ligand Interactions on Living Cells
23. Computer Simulation of Protein-Ligand Interactions: Challenges and Applications
24. Force Probe Molecular Dynamics Simulations
25. Study of Ligand-Protein Interactions by Means of Density Functional Theory and First-Principles Molecular Dynamics

P.D.: Al libro le faltan los capítulos 5 y 23. Los estoy buscando, cuando los tenga los subiré en esta misma entrada.

Actualización: 23/08/2012

Membrane Protein Protocols

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Contenidos del Libro:

1. Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli
2. Dihydroorotate Dehydrogenase of Escherichia coli
3. Protein Expression in Escherichia coli: The Uncoupling Protein, UCP1, as an Example
4. Expression of Membrane-Bound Iron-Sulfur Proteins
5. Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris
6. Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells
7. Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells
8. Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
9. Solubilization of Chemokine Receptors from Cell Membranes
10. A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid:Acyl-Coa Acyltransferase (LAT)
11. Purification of Omp50, a Minor Porin of Campylobacter jejuni
12. Purification of Porins from Mycobacterium smegmatis
13. Isolation of the Melanocortin 5 Receptor: From cDNA Sequence to Isolating an Integral Membrane Protein
14. Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography
15. Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells
16. Purification of Pancreastatin Receptor from Rat Liver Membranes
17. Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes
18. Isolation of Lipid Raft-Associated Proteolipids
19. Purification of Membrane-Bound Catechol-O-Methyltransferase
20. Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane
21. Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase: A Putative PS Flippase
22. Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes
23. Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I
24. Crystallization in Lipidic Cubic Phases: A Case Study with Bacteriorhodopsin
25. Optical Biosensor Assay Using Retroviral Receptor Pseudotypes

 Actualización: 23/08/2012

The Protein Protocols Handbook

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Contenidos del Libro:

1. Quantitation of Proteins
2. Electrophoresis of Proteins and Peptides and Detection in gels
3. Blotting and Detection Methods
4. Chemical Modification of Proteins and Peptide Production and Purification
5. Protein/Peptide Characterization
6. Glycoproteins
7. Antibody Techniques
8. Monoclonal Antibodies

Protein Purification

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Contenidos del Libro:

1. The Protein Purification Laboratory
2. Making an Extract
3. Analysis: Measurement of Protein and Enzyme Activity
4. Separation and Precipitation
5. Separation by Adsorption I: General Principles
6. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorvents
7. Separation by Adsorption: Affinities Techniques
8. Separation in Solution
9. Purification of Special Types of Proteins
10. Small-Scale and Large-Scale Procedures
11. Analysis for Purity
12. Optimization of Procedures: Final Steps