Biopharmaceuticals in Plants: Toward the Next Century of Medicine

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Contenidos del Libro:

1. History of Plants in Production of Biopharmaceuticals
2. Transgenic Plants Expressing Vaccine and Therapeutic Proteins
3. Chloroplast Engineering and Production of Biopharmaceuticals
4. Plant Viral Expression Vectors and Production of Biopharmaceuticals in Plants
5. Glycosylation of Therapeutic Proteins in Plants
6. Scale-Up of Plant-Derived Pharmaceuticals: Prospects for Commercial Production and for Global HealthThe Immune Response to Plant-Derived Pharmaceuticals
7. Risk Analysis and Safety of Plant-Made Biopharmaceuticals
8. Epilogue: The Future

Actualización: 22/09/2012

Receptor Signal Transduction Protocols

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Contenidos del Libro:

1. Radioligand-Binding Methods for Membrane Preparations and Intact Cells
2. Determination of Allosteric Interactions Using Radioligand-Binding Techniques
3. Generation, Use, and Validation of Receptor-Selective Antibodies
4. Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization
5. Generation and Use of Epitope-Tagged Receptors
6. Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization
7. Electron Microscopic Studies of Receptor Localization
8. Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
9. Viral Infection Protocols
10. Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection
11. Covalent Modification of G-Proteins by Affinity Labeling
12. Measurement of Agonist-Stimulated [35S]GTPγS Binding to Assess Total G-Protein and Gα-Subtype-Specific Activation by G-Protein-Coupled Receptors
13. Identification and Quantitation of G-Protein α-Subunits
14. Analysis of Function of Receptor–G-Protein and Receptor–RGS Fusion Proteins
15. Assessment of Receptor Internalization and Recycling
16. G-Protein-Coupled Receptor Phosphorylation and Palmitoylation
17. Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping
18. Ubiquitination of G-Protein-Coupled Receptors
19. Receptor Mutagenesis Strategies for Examination of Structure–Function Relationships
20. Study of G-Protein-Coupled Receptor–Protein Interactions by Bioluminescence Resonance Energy Transfer
21. Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells
22. Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation
23. Study of G-Protein-Coupled Receptor–Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase–Fusion Protein Pull-Downs
24. Receptor Knock-Out and Knock-In Strategies
25. Statistical Methods in G-Protein-Coupled Receptor Research

Actualización: 23/08/2012

Biochemistry of Signal Transduction and Regulation

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Contenidos del Libro:

1. The Regulation of Gene Expression
2. The Regulation of Enzyme Activity
3. Function and Structure of Signaling Pathways
4. Signaling by Nuclear Receptors
5. G-protein Coupled Signal Transmission Pathways
6. Intracellular Messenger Substances: “Second Messengers”
7. Ser/Thr-specific Protein Kinases and Protein Phosphatases
8. Signal Transmission via Transmembrane Receptors with Tyrosine-specific Protein Kinase Activity
9. Signal Transmission via Ras Proteins
10. Intracellular Signal Transduction: the Protein Cascades of the MAP Kinase Pathways
11. Membrane Receptors with Associated Tyrosine Kinase Activity
12. Other Receptor Classes
13. Regulation of the Cell Cycle
14. Malfunction of Signaling Pathways and Tumorigenesis: Oncogenes and Tumor Suppressor Genes
15. Apoptosis
16. Ion Channels and Signal Transduction

 Actualización: 20/09/2012

Signaling Through cell Adhesion Molecules

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Contenidos del Libro:

Section I: Protein-Protein Interactions and Early Events in Integrin Signaling
1.- The Use of Chimeric Receptors in the Study of Integrin Signaling
2.- Using Synthetic Peptides to Mimic Integrins: Probing Cytoskeletal Interactions and Signaling Pathways
3.- Use of the Yeast Two-Hybrid System for the Characterization of Integrin Signal Transduction Pathways: Identification of the Integrin-Linked Kinase, p59ILK
4.- Focal Adhesion Kinase and Its Associated Proteins
5.- Analysis of Paxillin as a Multi-Domain Scaffolding Protein
6.- P130Cas in Integrin Signaling
7.- Specificity of Integrin Signaling

Section II: Late Events and Biological Functions of Integrin Signaling
8.- Methods for Study of Integrin Regulation of MAP Kinase Cascades
9.- Methods for Analysis of Adhesion-Dependent Cell Cycle Progression
10.- Integrin Modulation of Mitogenic Pathways Involved in Muscle Differentiation
11.- Methods for Studying Anoikis
12.- Functional Analysis of FAK and Associated Molecules in Cell Migration
13.- Integrin Signaling in Pericellular Matrix Assembly

Section III: Inside-Out Signaling by Integrins
14.- Studies of Integrin Signaling Through Platelet α_IIbβ₃
15.- Tracking Integrin-Mediated Adhesion Using Green Fluorescent Protein and Flow Cytometry
16.- Expression Cloning of Proteins that Modify Integrin Activation
17.- Regulation of Cell Contractility by RhoA: Stress Fiber and Focal Adhesion Assembly

Section IV: General Methods for Signaling Studies of Cell Adhesion Molecules
18.- Intra- and Intercellular Localization of Proteins in Tissue In Situ
19.- Optical Microscopy Studies of [Ca2+]i Signaling
20.- Wnt Signaling in Xenopus Embryos

 Actualización: 20/09/2012

Smad Signal Transduction: Smad in Prolifetion, Differenciation and Disease

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Contenidos del Libro:

Preface: The Smad Family
1.- Molecular Evolution of Smad Proteins
2.- C. elegans TGF-β Signaling Pathways
3.- Smads in Drosophila – Interpretation of Graded Signals in vivo
4.- Delineating the TGF-β/Smad-induced Cytostatic Response
5.- Smads in Mesenchymal Differentiation
6.- Smad Proteins in Apoptotic and Survival Signaling
7.- TGF-β/Smad Signaling in Epithelial to Mesenchymal Transition
8.- Genetic Disruptions within the Murine Genome Reveal Numerous Roles of the Smad Gene Family in Development, Disease, and Cancer
9.- Trafficking of Serine/Threonine Kinase Receptors and Smad Activation
10.- Nucleocytoplasmic Shuttling of Smad Proteins
11.- Structural Insights into Smad Function and Specificity
12.- Regulation of Smad Function by Phosphorylation
13.- Regulation of Smad Functions through Ubiquitination and Sumoylation Pathways
14.- Smad Transcriptional Co-activators and Co-repressors
15.- Integration of Signaling Pathways via Smad Proteins
16.- Interplay between Smad and Map Kinase Signaling Pathways
17.- Gene Expression Signatures of TGF-β/Smad-Induced Responses
18.- Systems Biology Approaches to TGF-β/Smad Signaling
19.- Inhibitory Smads: Mechanisms of Action and Roles in Human Diseases
20.- Alterations in Smad Signaling in Carcinogenesis
21.- TGF-β Receptor Kinase Inhibitors for the Treatment of Cancer
22.- TGF-β Receptor Kinase Inhibitors for Treatment of Fibrosis

 Actualización: 20/09/2012

Signal Transduction

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Contenidos del Libro:

1. Prologue: Signal Transduction, Origins, and Ancestors
2. First Messengers
3. Receptors
4. GTP-binding Proteins and Signal Transduction
5. Effector Enzymes Coupled to GTP Binding Proteins: Adenylyl Cyclase and Phospholipase C
6. The Regulation of Visual Transduction and Olfaction
7. Intracellular Calcium
8. Calcium Effectors
9. Phosphorylation and Dephosphorylation: Protein kinases A and C
10. Nuclear Receptors
11. Growth Factors: Setting the Framework
12. Signalling Pathways Operated by Receptor Protein Tyrosine Kinases
13. Signal Transduction to and from Adhesion Molecules
14. Adhesion Molecules in the Regulation of Cell Diff erentiation: Mainly About Wnt
15. Activation of the Innate Immune System: the Toll-like Receptor 4 and Signalling through Ubiquitylation
16. Traffi c of White Blood Cells
17. Tyrosine Protein Kinases and Adaptive Immunity: TCR, BCR, Soluble Tyrosine Kinases and NFAT
18. Phosphoinositide 3-Kinases, Protein Kinase B, and Signalling through the Insulin Receptor
19. Protein Kinase C Revisited
20. Signalling Through Receptor Serine/ Threonine Kinases
21. Protein Dephosphorylation and Protein Phosphorylation
22. Notch
23. Targeting Transduction Pathways for Research and Medical Intervention
24. Protein Domains and Signal Transduction

Actualización: 20/09/2012

Handbook of Prebiotics and Probiotics Ingredients: Health Benefits and Food Applications

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Último libro sobre probioticos, a petición de Sahrigar.... mañana parto con Transducción de Señales a petición de Rafael

Contenidos del Libro:

1.-Analysis of Dietary Fiber and Nondigestible Carbohydrates

Part I: Sources of Prebiotics
2.- Short-Chain Fructo-Oligosaccharide: A Low Molecular Weight Fructan
3.- Inulin and Oligosaccharides: A Special Focus on Human Studies
4.- Galacto-Oligosaccharides
5.- Functional Disaccharides: Lactulose, Lactitol, and Lactose
6.- Natural Resistant Starches as Prebiotics and Synbiotics
7.- AGE, ALE, RAGE, and Disease: A Food Perspective

Part II: Sources of Probiotics
8.- Lactic Acid Bacteria and Plant Fibers: Treatment in Acute and Chronic Human Disease
9.- Probiotics: Recent Human Studies Using Lactobacillus casei strain Shirota

Part III: Physiological Functions of Prebiotics and Probiotics
10.- Prebiotics and Lipid Metabolism
11.- Fermentation of Prebiotics and Short-Chain Fatty Acid Production
12.- Probiotics and Prebiotics in Inflammatory Bowel Disease
13.- Prebiotics and Probiotics in Pediatric Diarrheal Disorders
14.- Anticarcinogenic Effects of Probiotics, Prebiotics, and Synbiotics
15.- Prebiotics and Probiotics in Infant Formulae
16.- Probiotics and Prebiotics in Elderly Individuals
17.- Prebiotics and Probiotics in Companion Animal Nutrition
18.- Probiotics: Potential Pharmaceutical Applications

Probiotics in Food Safety and Human Health

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Contenidos del Libro:

1. Introduction to Prebiotics and Probiotics
2. Development of Probiotic Food Ingredients
3. Taxonomy and Biology of Probiotics
4. Primary Sources of Probiotic Cultures
5. Properties of Evidence-Based Probiotics for Human Health
6. Genetic Engineering of Probiotic Bacteria
7. Immunochemical Methods for Detection of Probiotics
8. Molecular-Based Methods Directed toward Probiotics
9. Application of Repetitive Element Sequence-Based (rep-) PCR and Denaturing Gradient Gel Electrophoresis for the Identification of Lactic Acid Bacteria in Probiotic Products
10. Genetically Modified Probiotics
11. Use of Probiotics in Preharvest Food Safety Applications
12. Preharvest Food Safety Applications of Competitive Exclusion Cultures and Probiotics
13. Probiotics as Biopreservatives for Enhancing Food Safety
14. EU Perspectives on Food, Gastrointestinal Tract Functionality, and Human Health
15. Modulation of Epithelial Function and Local Immune System by Probiotics: Mechanisms Involved
16. Probiotics in Cancer Prevention
17. Statistical Considerations for Testing the Efficacy of Probiotics
18. The Safety of Probiotics in Foods in Europe and Its Legislation
19. Probiotics in Food Safety and Human Health: Current Status of Regulations on the Use of Probiotics in Foods in Japan

Probiotics: Biology, Genetics and Health Aspects

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Contenidos del Libro:

1. Biology of Prokaryotic Probiotics
2. Biology of Eukaryotic Probiotics
3. Detection and Identification of Probiotic Microorganisms and Other Beneficial Organisms from the Human GI Tract
4. Maintenance and Protection of Probiotics
5. Genetic Engineering of Probiotic Microorganisms
6. Roles of Probiotic on Gut Health
7. New Health Potentials of Orally Consumed Probiotic Microorganisms
8. Carriers of Probiotic Microorganisms
9. Use of Probiotics for Dermal Applications
10. Challenges of Beneficial Health Claims
11. Safety and Regulation of Probiotic Foods and Supplements
12. Current Market Trends and Future Directions

Probiotic Bacteria and Enteric Infections: Cytoprotection by Probiotic Bacteria

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Libro a petición de Sahrigar..... se vienen más.

Contenidos del Libro:

Part I: Introduction and History of Probiotics
1.- Probiotics: From the Ancient Wisdom to the Actual Therapeutical and Nutraceutical Perspective

Part II: The Gut Microorganisms and Probiotics
2.- The Intestinal Microbiota and Probiotics
3.- Ecology of Probiotics and Enteric Protection

Part III: Pathophysiology of Enteric Disorders Due to Disturbed Microbiota
4.- Factors Causing Disturbances of the Gut Microbiota
5.- The Gut Microbiota, Probiotics and Infectious Disease

Part IV: Application of Molecular Biology and -omics of Probiotics in Enteric Protection
6.- Application of Molecular Biology and Genomics of Probiotics for Enteric Cytoprotection
7.- Application of Probiotic Proteomics in Enteric Cytoprotection
8.- Promoting Gut Health with Probiotic Metabolomics

Part V: Probiotics, Gut Immunology and Enteric Protection
9.- Microbiota, Probiotics and Natural Immunity of the Gut
10.- Interaction of Probiotics with the Acquired Immune System in the Gut
11.- The Protective Role of Probiotics in Disturbed Enteric Microbiota
12.- Modulation of Immune System by Probiotics to Protect Against Enteric Disorders

Part VI: Probiotics for Enteric Therapy
13.- Probiotic-Pathogen Interactions and Enteric Cytoprotection
14.- Bacteriocins of Probiotics and Enteric Cytoprotection
15.- Probiotics in Clinical Practice as Therapeutics Against Enteric Disorders
16.- Potential Mechanisms of Enteric Cytoprotection by Probiotics: Lessons from Cultured Human Intestinal Cells
17.- Probiotics and Enteric Cancers

Part VII: The Future of Probiotics
18.- Designer Probiotics and Enteric Cytoprotection
19.- Future Prospects of Probiotics as Therapeutics Against Enteric Disorders

Electron Microscopy: Principles and Fundamentals

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Contenidos del Libro:

Part I: Stationary Beam Methods

1.- Transmission Electron Microscopy

2.- Reflection Electron Microscopy

3.- Electron Energy-Loss Spectroscopy Imaging

4.- High Voltage Electron Microscopy

5.- Convergent Beam Electron Diffraction

6.- Low-Energy Electron Microscopy

7.- Lorentz Microscopy

8.- Electron Holography Methods

9.- Spin-Polarized Low-Energy Electron Microscopy

Part II: Scanning Beam Methods

1.- Scanning Reflection Electron Microscopy

2.- Scanning Transmission Electron Microscopy

3.- Scanning-Transmission Electron Microscopy : Z Contrast

4.- Scanning Auger Microscopy (SAM) and Imaging X-Ray Photoelectron Microscopy (XPS)

5.- Scanning Microanalysis

6.- Imaging Secondary Ion Mass Spectrometry

7.- Scanning Electron Microscopy with Polarization Analysis (SEMPA)

Actualización: 23/08/2012

Söderhäll - Invertebrate Immunity

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Contenidos del Libro:

1. Cnidarian immunity: a tale of two barriers
2. Gastropod immunobiology
3. Bivalve immunity
4. Earthworm immunity
5. Leech immunity: from brain to peripheral responses
6. Innate immunity in C. elegans
7. Immunocompetent molecules and their response network in horseshoe crabs
8. Tick innate immunity
9. Beetle immunity
10. Immunity in lepidopteran insects
11. Fly immunity: recognition of pathogens and induction of immune responses
12. Mosquito immunity
13. Crustacean immunity
14. Echinoderm immunity
15. Urochordate immunity

Algal Culturing Techniques

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Libro aportado por Leonardo.... Muchas gracias!

Contenidos del Libro:

1. Historical Review of Algal Culturing Techniques
2. Freshwater Culture Media
3. Marine Culture Media
4. Trace Metal Ion Buffers and Their Use in Culture Studies
5. Sterilization and Sterile Technique
6. Traditional Microalgae Isolation Techniques
7. Automated Isolation Techniques for Microalgae
8. Purification Methods for Microalgae
9. Isolation and Purification Techniques for Macroalgae
10. Perpetual Maintenance of Actively Metabolizing Microalgal Cultures
11. Long-Term Macroalgal Culture Maintenance
12. Cryopreservation Methods for Maintaining Microalgal Cultures
13. Photobioreactors and Fermentors: The Light and Dark Sides of Growing Algae
14. Culturing Microalgae in Outdoor Ponds
15. Mariculture of Seaweeds
16. Counting Cells in Cultures with the Light Microscope
17. Phytoplankton Cell Counting by Flow Cytometry
18. Measuring Growth Rates in Microalgal Cultures
19. Using Cultures to Investigate the Physiological Ecology of Microalgae
20. Analysis of Algal Pigments by High-Performance Liquid Chromatography
21. Endogenous Rhythms and Daylength Effects in Macroalgal Development
22. Viral Contamination of Algal Cultures
23. Control of Sexual Reproduction in Algae in Culture
24. Microalgal Life Cycles: Encystment and Excystment
25. Cultures as a Means of Protecting Biological Resources: Ex Situ Conservation of Threatened Algal Species

Appendix A: Recipes for Freshwater and Seawater Media

Actualización: 22/09/2012